Past Questions Archive - Western Delta University, Oghara Delta State

first semester examination 2023/2024 session

BCH209 - practical biochemistry time: 1hr

answer all questions

Section A
1. Name the test to confirm the presence of carbohydrates in samples.
2. Benedict's reagent is used to detect the presence of _______ sugars.
3. Hopkin's Cole glyxylic reaction confirms the presence of _______.
4. Pentoses, when boiled in the presence of hydrochloric acid, dehydrate, yield aldehydes of furfural type, which condense with orcinol to form _______ coloured compound.
5. When carbohydrates are boiled with excess of phemythdrazine, and additional condensation takes place, forming _______.
6. _______ test is used to confirm the presence of reducing monosaccharides.
7. Fearson's test confirms the presence of _______.
8. _______ is used to detect the presence of sulphur amino acids.
9. The presence of arginine in samples is detect using _______ test.
10. What indicator is used for Sørensen's formol Titration?
11. Starch is confirmed using _______ test.
12. The principle of _______ test is that the aldehyde or ketone group of the carbohydrates reduces blue cupric hydroxide to an insolubble yellow to red cuprous oxide.
13. The reagents used for Sørensen's formol Titration is formaladehyde and _______.
14. Phenols and compounds containing the hydroxybenzene radical react with _______ reagent to form red complexes.
15. Molish reagent contains _______ in ethanol.
Section B
1. Question 1
  List five precautions when carrying out biochemical tests.
2. Question 2
  How can you distinguish between fructose and sucrose.

Western Delta University, Oghara Delta State